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. 2016 Jul 9;7(50):82213–82227. doi: 10.18632/oncotarget.10511

Figure 4. BARF1-mediated down regulation of nuclear SMAD4, and the effect of SMAD4 on cell proliferation.

Figure 4

(A) Immunofluorescence was visualized via confocal microscopy. BARF1 attenuated nuclear SMAD4 in SNU601 BARF1 cells, whereas BARF1 inhibition augmented nuclear SMAD4 in YCCEL1 cells transfected with siBARF1. Cells were labeled with SMAD4 antibody (green), and nuclei were counterstained with DAPI (blue). (B) Western blotting of nuclear extracts confirmed the BARF1-mediated downregulation of nuclear SMAD4 (*P < 0.05). TFII B was used as a loading control. (C) SNU601 BARF1 and SNU601 mock cells were transfected with pCMV-SMAD4 (SMAD4) or pCMV-empty vector (VECTOR). Cell proliferation was suppressed by pCMV-SMAD4 transfection compared with cells transfected with empty vector (**P < 0.01). All experiments were performed in triplicate.