Figure 5. c-Myc target gene regulation in IMEC.
(A) IMEC/vec were transfected with c-Myc siRNA or non-targeting control for 72hrs. Protein analysed by Western blotting. (B) The expression of c-Myc target gene transcripts analysed by RT-qPCR, relative to GAPDH. (C) IMEC/vec were transfected with non-targeting control, c-Myc siRNA or CE siRNA for 72hrs. Protein analysed by Western blotting. Significance calculated relative to control siRNA using Student's T-test, ** = p≤0.01; * = p≤0.05.