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. 2016 Oct 16;7(50):82273–82288. doi: 10.18632/oncotarget.12701

Figure 6. c-Myc depletion reduces target genes dependency on CE.

Figure 6

(A) HeLa cells were transfected with CE siRNA, c-Myc siRNA and/or non-targeting control for 72hrs. Protein analysed by Western blotting. (B) Densitometry performed to quantify the decrease in c-Myc Western blot signal (normalised to actin), in response to CE depletion in HeLa cells, transfected with c-Myc siRNA or non-targeting control. Error bars represent standard error of the mean, n≥6. (C) c-Myc siRNA, CE siRNA and control transfections were performed for 72 hours. c-Myc target gene transcripts were analysed by RT-qPCR, normalised to GAPDH. Error bars represent standard error of the mean, n=4. (D) Average change in c-Myc target gene transcripts (NCL, NME1, ODC, FBL and NPM) in response to CE siRNA, relative to control transfections. Error bars represent standard error of the mean, n=5. (E) Decrease in c-Myc Western blot signal following c-Myc and/or CE knockdown, relative to control transfection. Error bars represent standard error of the mean, n≥6. (F) Average decrease in c-Myc target gene transcripts (NCL, NME1, ODC, FBL and NPM) following c-Myc and/or CE knockdown, relative to control transfection. Error bars represent standard error of the mean, n=5. Significance was calculated by Student's T-test, ***= p≤0.001; **= p≤0.01; *= p≤0.05. For B and D-F, significance relative to control siRNA.