Figure 4. Targeting serpinE2 with Ab11 reduces lung metastasis in breast cancer models.

(A) Tumor volume 7 weeks after orthotopic injection of MDA-MB435 cells into mammary fat pads of SCID mice. Mice were treated with control IgG or Ab11 dosed at 30mg/kg; at least 3 mice per group; n.s. = no significance. (B) MDA-MB435 tumors were harvested from mice treated with IgG or Ab11 3 hours before dissection, and were fixed and analyzed by IHC for ERK1/2 phosphorylation (brown staining; representative images from 3 tumors per treatment condition). Small panel shows the “no primary antibody” control staining. (C) Spontaneous metastatic potential of tumor-bearing mice from (A) was determined by quantifying visible metastatic nodules on the surface of the lungs. 6-8 animals in the indicated treatment groups. * P < 0.0137. (D) Tumor volume 23 days after orthotopic injection of 4T1 cells into mammary fat pads of Balb/c mice treated with control IgG +Vehicle, Ab11+Vehicle, control IgG + dovitinib, or Ab11+ dovitinib, as indicated. 6-8 tumors per group. *P < 0.0057, ** P < 0.0016. (E) 4T1 tumors were harvested from mice treated with either IgG or Ab11 as in (B) and were analyzed by IHC for ERK1/2 phosphorylation (brown staining; representative images from 3 tumors per treatment condition). Small panel shows the “no primary antibody” control staining. (F) Spontaneous metastatic potential of tumor-bearing mice from (D) was determined by quantifying visible metastatic nodules on the surface of the lungs. Data are means ± SEM of counts from at least 4 animals carrying tumors from the indicated groups. * P < 0.05. Images are from 10X magnification, scale bars = 200 μm. Data are shown as means ± SEM.