Figure 2. Irradiation did not recruit peripheral monocytes into the hippocampus.

No CCR2^RFP/+-labeled cells were detected in the hippocampus at any of the time points after IR, neither in juvenile nor in adult brains. Scale bar, 150 μm (A). Unlike after IR, infiltrating monocyte-derived macrophages (CCR2^RFP/+-labled cells, red) appeared in the injured hippocampus already 1 day after an ischemic insult (B., right panel), in contrast to the uninjured hippocampus where only CX3CR1^GFP/+-labeled cells (green) were detected (B, left panel). Scale bar, 100 μm. DG = dentate gyrus, GCL = granule cell layer.