Skip to main content
. 2016 Jun 30;7(50):82324–82337. doi: 10.18632/oncotarget.10327

Figure 4. Assessment of the growth and invasive advantages provided by senescent fibroblasts to LCC cells in culture.

Figure 4

A. Outline of the experimental design used to assess growth (top right) and invasion (bottom right) in cancer cells in culture stimulated with conditioned medium (CM) from fibroblasts co-cultured as in Figure 2A. B, C. Average cell number/field of either (B) H460 LCC cells or (C) A549 ADC cells cultured with different types of conditioned medium as described in (A). Results were normalized to control medium, and are shown as mean ± SE. D, E. Average invasion of either (D) H460 cells or (E) A549 cells induced by different types of conditioned medium as in (B,C) assessed by area quantification of crystal violet positive invasive cells (top images). Results were normalized to control medium. All results correspond to at least two replicates from three independent experiments. All pair-wise comparisons were performed with respect to control medium unless otherwise indicated.