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. 2016 Aug 2;7(50):82338–82353. doi: 10.18632/oncotarget.11001

Figure 2. Overexpression of miR-891b significantly inhibited the proliferative capacity of PDAC cells.

Figure 2

(A) Following miR-891b transfection fold changes in the miR-891b levels were determined through qRT-PCR. U6 small nuclear RNA was used as an internal control for relative quantitation. *P < 0.05. (B) PDAC cell lines, SW1990 and PANC-1, were transfected with miR-891b mimic or NC. Cells were collected 24, 48, 72, and 96 h after the transfection. The proliferation curve was drawn by using the trypan blue cell counting method. The results suggested miR-891b significantly inhibited the proliferation of PDAC cells (mean ± SD, results of three independent experiments, *P < 0.05). (C) Establishment of a nude mouse model of PDAC subcutaneous transplantation tumor with SW1990 cell line. MiR-891b agomir was intratumorally injected after the tumor was formed. After 2 weeks, compared with NC-treated tumor, the size of the subcutaneous tumor treated with miR-891b agomir significantly decreased. (D) The volume of the subcutaneous tumor was measured regularly during the period of the injection to draw the growth curve of the tumor. The results suggested that compared with NC-treated tumors the volume of miR-891b agomir-treated tumors decreased significantly. The error line represents the mean ± SD, *P < 0.05. (E) The nude mice were killed after 2 weeks of treatment. The tumors were completely dissected and weighted. The weight of the tumor treated with miR-891b agomir decreased significantly compared with tumor treated with NC. The error line represents the mean ± SD, *P < 0.05. (F) The expression of Ki-67 protein in human PDAC tissue samples was detected using an immunohistochemical assay. The expression of miR-891b was evaluated by qRT-PCR. The relative quantitation of miR-891b was expressed as medians with interquartile (P25–P75). The comparisons between the expression of miR-891b and Ki-67 protein were performed with a Mann-Whitney U test. The results showed that the expression of miR-891b significantly varied with the expression of Ki-67.