Figure 4. The expression of Cbl-b was associated with the proliferative capacity of PDAC cells.

(A) Silencing the expression of Cbl-b significantly inhibited the proliferative capacity of PDAC cells. The PDAC cell lines, SW1990 and PANC-1, were transfected with Cbl-b siRNA or NC. Cells were collected 24, 48, and 72 h after transfection. The proliferation curve was drawn using the trypan blue cell counting method. The results suggested that silence of Cbl-b significantly inhibits the proliferation of PDAC cells (mean ± SD, results of three independent experiments, *P < 0.05). (B) The expression of Cbl-b and Ki-67 in PDAC tissues was detected using immunohistochemistry. a, c and e: the expression of Cbl-b in PDAC tissue. Positive expression shows brown-yellow particles distributed in the membrane and cytoplasm of the cells (SP staining × 200). b, d and f: the expression of Ki-67 in PDAC tissue was detected. Positive expression shows brown-yellow particles distributed in the nuclei of the cells (SP staining × 200). (C) The expression of Cbl-b protein in human PDAC tissue samples was detected using an immunohistochemical assay. The expression of miR-891b was evaluated by qRT-PCR. The relative quantitation of miR-891b was expressed as medians with interquartile (P₂₅–P₇₅). The comparisons between the expression of miR-891b and Cbl-b protein were performed with a Mann-Whitney U test. The results showed that the expression of miR-891b significantly varied with the expression of Cbl-b.