Figure 6. RND3 negatively regulated the expression levels of Snail protein but not the transcript.

(A) Immunoblot analysis showed that Rnd3 deficiency resulted in increases in the Snail at protein levels in Rnd3 null mouse brain tissues. (B) No changes of Snail1 transcripts were found in the same cohort of Rnd3 null mouse brains. (C) Forced expression of RND3 in U251 cells resulted in a decrease in Snail1 protein level. An opposite result was observed when RND3 was knocked down. (D) The transcript levels of Snail1 remained unchanged under overexpression and downregulation of RND3 conditions. Student's t was used for the statistical analyses.