Figure 5. Hsp90α deficient cells have reduced γH2AX.

(A) Mouse embryonic fibroblasts that have wild-type Hsp90α and that are Hsp90α null were radiated with 10 Gy, lysed at various time points, and immunoblotted with the indicated antibodies. (B) UMSCC1 cells were transfected with a 50 nM concentration of non-specific siRNA or Hsp90α siRNA, irradiated with 4 Gy 48 hours following the transfection, lysed at the 10, 30, and 60 minute time points, and immunoblotted with the indicated antibodies.