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. 2016 Oct 15;7(50):82493–82503. doi: 10.18632/oncotarget.12696

Figure 1. Auto-antibodies against FIR/FIRΔexon2 purified proteins were detected in the sera of colorectal cancer patients.

Figure 1

(A) Expression of FIRs proteins were examined by western blotting in tumor (T) and adjacent non-tumor (N) tissue samples from colon cancers and colon polyps' tissues. Representative cases were indicated. (B) Bands' intensities were quantified using Scion Image imaging analysis software (National Institutes of Health. USA) and the average band intensity of proteins normalized to the corresponding β-actin were shown. (C) FIR and FIRΔexon2 purified proteins were prepared as antigens to detect the auto-antibodies against FIR/FIRΔexon2 in colorectal cancer patients' sera by western blotting (upper panel). The anti-FIRs (6B4) antibody was used as a positive control (middle panel). Molecular weight of bands detected by patients' sera were exactly same size with those detected by anti-FIRs (6B4) antibodiy in western blot (middle panel) shown in CBB (Coomassie Brilliant Blue) staining (lower panel). (D) Auto-antibodies against FIR or FIRΔexon2 in colorectal cancer patients' sera were further confirmed in dose dependent manner by western blotting. FIRwt: FIR purified protein. BSA: Bovine serum albumin.