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. 2016 Oct 20;7(50):82554–82566. doi: 10.18632/oncotarget.12783

Figure 2. BBD attenuated hepatic fibrosis.

Figure 2

4-μm-thick paraffin-embedded liver section was using for histopathological examination. (A) The deposition of collagens was stained by Masson'trichrome (× 200) and the stained area was quantified using an image analysis system (B). (C) The contents of hydroxyproline, one of the main content of collagens, were tested according the protocol in Hydroxyproline Testing Kit. (D) Sirius red (× 100) was stained for hepatic fibrosis. (E) The percentage of Sirius-red in fibrotic livers was quantified using an image analysis system. Compared to DEN group, *P < 0.05; **P < 0.01, compared to Normal group, NS: no significance, by two-tailed Student's t test, n = 8, bar = 1 mm.