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. 2016 Oct 20;7(50):82554–82566. doi: 10.18632/oncotarget.12783

Figure 4. BBD-serum inhibited the LPS-induced HSCs activation.

Figure 4

Primary HSC was isolated and cultured with LPS and different gradient concentration of BBD-serum for 72 h. (A) Immunofluorescence staining was performed to identify the activated HSCs by α-SMA (× 200), bar = 100 um and the mRNA of α-SMA was determined by RT-PCR and western blot (B and C). (D) The collagen and inflammatory cytokines secreted by HSCs were also determined by RT-PCR. Compared to 10% N with LPS group, *P < 0.05; **P < 0.01, by two-tailed Student's t test, N: normal-serum; B: BBD-serum.