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. 2016 Oct 20;7(50):82554–82566. doi: 10.18632/oncotarget.12783

Figure 5. BBD-serum inhibited the LPS-induced HSCs activation by blocking NF-κB signaling pathway.

Figure 5

Primary HSC was isolated and cultured with LPS and different gradient concentration of BBD-serum for 72 h. (A) Immunofluorescence staining was performed to identify the expression of TLR4 (× 200), bar = 100 um and the mRNA of TLR4 was determined by RT-PCR (B). (C) Downstream genes of TLR4 were detected by RT-PCR. (D) Quantitative analysis of the Western blots for IKK and IκBα. Compared to 10% N. with LPS group, *P < 0.05; **P < 0.01, by two-tailed Student's t test; N: normal-serum; B: BBD-serum.