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. 2016 Nov 2;7(50):82620–82633. doi: 10.18632/oncotarget.13012

Figure 2. Silencing PVT1 by siRNA inhibits proliferation and promotes apoptosis in osteosarcoma cells.

Figure 2

(A) qRT-PCR was used to measure the expression level of PVT1 in U2OS and MG-63 cells that had been transfected with control or siRNAs against PVT1, i.e., si-PVT1#1, si-PVT1#2, or si-PVT1#3 (*P < 0.05). (B–C) Cell proliferation was assessed with an MTT assay in U2OS and MG-63 cells transfected with control or si-PVT1#1 (*P < 0.05). (D–E) Cell apoptosis was assessed by Annexin-V/7-AAD staining in U2OS and MG-63 cells transfected with control or si-PVT1#1 (*P < 0.05). (F) An immunofluorescence assay was performed to measure TUNEL expression in U2OS and MG-63 cells transfected with control or si-PVT1#1. (G–H) A clonal colony-forming assay was performed to assess cell proliferation in U2OS and MG-63 cells transfected with control or si-PVT1#1 (*P < 0.05).