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. 2016 Nov 2;7(50):82620–82633. doi: 10.18632/oncotarget.13012

Figure 5. Silencing PVT1 by siRNA suppresses proliferation, migration and invasion and promotes cell cycle arrest and apoptosis via miR-195 in osteosarcoma cells.

Figure 5

(A) U2OS and MG-63 cells were transfected with control and anti-NC, si-PVT1#1 and anti-NC, or si-PVT1#1 and anti-miR-195. The mRNA expression level of miR-195 was detected by qRT-PCR (*P < 0.05). (B–C) Cell proliferation was assessed with an MTT assay in U2OS and MG-63 cells treated as described in A (*P < 0.05). (D) A clonal colony-forming assay was performed to assess the proliferation of U2OS and MG-63 cells treated as described in A (*P < 0.05). (E) Apoptosis was assessed by Annexin-V/7-AAD staining in U2OS and MG-63 cells treated as described in A (*P < 0.05). (F) The invasion of U2OS and MG-63 cells treated as described in A was assessed using a Transwell assay (*P < 0.05). (G) The migration of U2OS and MG-63 cells treated as described in A was assessed using a Transwell assay (*P < 0.05). (H–I) The cell cycle distribution was analyzed by flow cytometry in U2OS and MG-63 cells treated as described in A.