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. 2016 Oct 13;7(50):82757–82769. doi: 10.18632/oncotarget.12634

Figure 6. Intact p53-MDM2 axis is required for the effect of SAR405838 on NB cells.

Figure 6

A. SH-SY5Y-vector and SH-SY5Y-p53-knockout cells were treated with10 μM SAR405838 for 0, 6, 12 hours, respectively. The cells were then lysed and subjected to SDS-PAGE, followed by immunoblotting with immunoblotting with the antibodies indicated. The α-Tubulin antibodies were used as a loading control for whole cell extracts. B. SH-SY5Y-vector and SH-SY5Y-p53-knockout cells were treated with 10 μM SAR405838 for 0 or 12 hours, respectively and collected for total RNA extraction. The relative RNA level for each gene indicated was measured by RT-PCR analysis.