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. 2016 Nov 7;7(50):82770–82782. doi: 10.18632/oncotarget.13165

Figure 3. LncRNA AF147447 over-expression inhibit GC proliferation and invasion in vitro and in vivo.

Figure 3

(A) Af147447 expression level after AF147447 over-expression was validated by qRT-PCR. (B) 7901 cells were seeded in 96-well plates, and cell proliferation was assessed daily for 3 days using the Cell Counting Kit-8 (CCK-8) assay. (C) Cells were treated with either pcDNA-AF147447 or pLV-AF147447 for 24 h. The representative images of invasive cells at the bottom of the membrane stained with crystal violet were visualized as shown. All experiments were performed in triplicate. (D) The in vivo models used were xenograft-transplanted nude mouse tumor models of human gastric cancer established with pLV-AF147447 or pLV-NC. The left arms of the mice were injected with pLV-AF147447 GC cells and the right arms of the mice were injected with pLV-NC cells. Photographs of tumors are presented. Effects of lncRNA AF147447 expression in nude mouse models are shown. (*p < 0.05, **p < 0.01).