Skip to main content
. 2016 Nov 7;7(50):82770–82782. doi: 10.18632/oncotarget.13165

Figure 4. Identification and validation of lncRNA-AF147447 targets.

Figure 4

(A) Biotinylated lncRNA-AF147447 or antisense RNA were incubated with nuclear extracts, targeted with streptavidin beads, and washed, and associated proteins were resolved in a gel. Western blotting analysis of the specific association of MUC2 and lncRNA-AF147447. Another MUC family: MUC1 is shown as a control. (B) Diagram of MUC2 promoter region constructs. (C) Luciferase reporter assays in 7901 cells, with co-transfection of wt or mt and lncRNA as indicated. (D) MUC2 expression was validated by qRT-PCR after transfecting with pLV-AF147447 or siRNA or their respective controls. (E) MUC2 expression was validated by western blot after transfecting with pLV-AF147447 or siRNA or their respective controls. (*p < 0.05; **p < 0.01).