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. 2016 Nov 7;7(50):82770–82782. doi: 10.18632/oncotarget.13165

Figure 7. LncRNA AF147447 physically associates with E2F1.

Figure 7

(A) RIP experiments were performed using the E2F1 antibody to immunoprecipitate (IP) and a primer to detect lncRNA-AF147447. RIP enrichment was determined as RNA associated with E2F1 IP relative to an input control. (B) Diagram of lncRNA-AF147447 promoter region constructs. Luciferase reporter assays in 7901 cells, with co-transfection of lncRNA-AF147447, with siRNA-E2F1 or siRNA-control. (C) LncRNA-AF147447 expression was detected by qRT-PCR after cells transfecting with si-E2F1 or pcDNA-E2F1. (D) MUC2 mRNA and protein were detected by qRT-PCR and western blot. (*p < 0.05; **p < 0.01).