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. 2016 Oct 14;7(50):82876–82888. doi: 10.18632/oncotarget.12657

Figure 3. Dasatinib suppresses LYN activity.

Figure 3

A. LYN tyrosine (Y396) phosphorylation levels were quantified using ELISA in dasatinib-treated ADC cells. Cells were treated with dasatinib at 2 different concentrations (DMSO as a control, IC50 x 0.1, and IC50). Median fluorescence intensity (MFI) values indicate levels of active pY396-LYN (n = 3; *, P < 0.05 vs. DMSO; #, P < 0.05 vs. IC50 x 0.1). B. Activated pY396-LYN levels were also measured by immunoprecipitating lysates from H358 and H1975 cells with an LYN antibody and immunoblotting with a cross-reactive pY416-SRC antibody. Cells were treated with dasatinib at 3 concentrations, and activated AKT and MAPK levels were determined using western blots. C. Cells were treated with dasatinib (DMSO as a control, IC50 × 0.1) 24 hrs after siRNA-mediated LYN knockdown. The effect of LYN knockdown on cell proliferation was analyzed using the WST-1 assay (n = 3; bars, SE; *, P < 0.05; Student's t-test). D. Cells were treated with dasatinib (DMSO as a control, IC50) after LYN knockdown. The effect of LYN depletion on cell migration was determined using a transwell assay.