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. 2016 Oct 17;7(50):82902–82920. doi: 10.18632/oncotarget.12703

Figure 3. PRRSV-vsRNA1 exhibits antiviral activity in different PRRSV strains in vitro.

Figure 3

MARC-145 cells were transfected with 100 nM of PRRSV-vsRNA1 mimics or NC mimics and then infected with 0.01 MOI of either rHP-PRRSV/SD16/EGFP or rPRRSV/CH-1a/DsRed PRRSV for 48 h. The percentage of EGFP- A. or DsRed- B. positive cells infected with PRRSV were measured by flow cytometry. PAM cells were transfected with PRRSV-vsRNA1 mimics or NC mimics (100 nM) and then infected with either 0.01 MOI of either highly pathogenic PRRSV strain GD-HD or low pathogenic PRRSV strain CH-1a. Expression levels of PRRSV ORF7 gene in PAM cells was evaluated by qRT-PCR C, D. Growth kinetics of PRRSV was evaluated by determination of the TCID50 E, F. Results are expressed as mean ± SD of three independent experiments. *p <0.05, **p <0.01, ***p < 0.001.