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. 2016 Oct 17;7(50):82902–82920. doi: 10.18632/oncotarget.12703

Figure 7. PRRSV-vsRNA1 targets PRRSV genomic RNA.

Figure 7

A. The 5 predicted PRRSV genome binding regions of PRRSV-vsRNA1 were cloned into the 3′UTR-luciferase reporter gene of psiCheck-2. B. Luciferase reporter plasmids psiCheck2-nsp1α, psiCheck2-nsp2, psiCheck2-nsp3, psiCheck2-nsp9, or psiCheck2-nsp10 were co-transfected with PRRSV-vsRNA1 mimics or NC mimics. Reporter activities were determined at 36 h post-transfection by dual-luciferase assays. C, D. RNA of mock- and GD-HD-infected PAM cells were subjected to Ago2-IP and the relative expression levels of PRRSV-vsRNA1(C) and NSP2 gene (D) in the immunoprecipitates were determined by qRT-PCR. As a negative control, immunoprecipitation was performed using IgG beads. **p <0.01, ***p < 0.001.