Table 1. Predicted microRNA in the PRRSV strain GD-HD genome.

Predicted microRNA	Mature sequence	Precursor sequence	Minimum free energy prediction (MFE)	Free energy of the thermodynamic ensemble
PRRSV-vsRNA1	UGCUGACUUG GCGCAACACGU	GUGAUGCGUCCA AGCUUAGUGAUC CUGCUACGCAGG AGUGGCUCU CUCGCAUGUGGGA UAGGGUUGACA UGCUGACUUGG CGCAACACGU	−35.40kcal/mol	−36.77 kcal/mol
PRRSV-vsRNA2	GGCGGGUGG GGCGAUGGUGGC	CUCAGCCUUAUGA UCCCAACCAGGCCG UAAAGUGCUUGCGG GUAUUACAGGCGG GUGGGGCGAU GGUGGCCGA	−25.00 kcal/mol	−27.00 kcal/mol
PRRSV-vsRNA3	AGAGGACGGCG CCAUUACUAU	GUCAGGUAUGGACAA GUCCUCACCCCCUAC CACAGGGACCGAGAGG ACGGCGCCAUU ACUAUCGAC	−21.90 kcal/mol	−22.85 kcal/mol
PRRSV-vsRNA4	UCUUGCCGCU AUUGAAGCCGA	GGCUACAUUGUCUCGCAU UAGUGGUUUGGAUGUGGUG GCUCACUUUCAACAUCU UGCCGCUAUUGAA GCCGAGACUUGUA	−28.20 kcal/mol	−29.19 kcal/mol