Table 3. Mimics sequence and primer used for psiCheck2 vector construction.
| Name | Sequence(5'-3′) |
|---|---|
| PRRSV-vsRNA1-mimics | UGCUGACUUGGCGCAACACGU |
| PRRSV-vsRNA1-MUT-mimics | UCAUAGUCUCUCGCAACACGU |
| NC-mimics PRRSV-vsRNA1-inhibitor NC-inhibitor |
AGCUGAUUUCGUCUUGGUA ACGUGUUGCGCCAAGUCAGCA UCACCGGGUGUAAAUCAGCUUGAC |
| psiCheck2-nsp1α-F | CCGCTCGAGATGTCTGGGATACTTGATCGGTGCAC |
| psiCheck2-nsp1α-R | TTGCGGCCGCTCTGCGGGAGCGGCAAGTTGGTTAAC |
| psiCheck2-nsp2-F | GCGATCGCGCCGGAAAGAGAGCAAGGAAAACACG |
| psiCheck2-nsp2-R | GGGTTTAAACCCGCCCAGTAACCTGCCAAGAATGGCAA |
| psiCheck2-nsp3-F | CCGCTCGAGGGGGCACGCTACATCTGGCACTTT |
| psiCheck2-nsp3-R | TTGCGGCCGCTCTCAAGGAGGGACCCGAGCTGAGA |
| psiCheck2-nsp9-F psiCheck2-nsp9-R |
CCGCTCGAGGGAGCAGTGTTTAAACTGCTAGCCGC TTGCGGCCGCTCTCATGATTGGACCTGAGTTTTTCCC |
| psiCheck2-nsp10-F | CCGCTCGAGGGGAAGAAGTCCAGAATGTGCGGGTA |
| psiCheck2-nsp10-R | TTGCGGCCGCTTTCCAGGTCTGCGCAAATAGCGCGGA |
| psiCheck2-PRRSV-1666-WT-F | TCGAGGACAGGAACGGCGCTTGCGGTAGCGCCAAGTACGTGCTTAAACTGGAGGGTGGC |
| psiCheck2- PRRSV-1666-WT-R | GGCCGCCACCCTCCAGTTTAAGCACGTACTTGGCGCTACCGCAAGCGCCGTTCCTGTCC |
| psiCheck2- PRRSV-3249-WT-F | TCGAGATCATGCGTGAGGCTTGTGATGCGTCCAAGCTTAGTGATCCTGCTACGCAGGGC |
| psiCheck2- PRRSV-3249-WT-R | GGCCGCCCTGCGTAGCAGGATCACTAAGCTTGGACGCATCACAAGCCTCACGCATGAC |
| psiCheck2- PRRSV-1666-MUT-F | TCGAGGACAGGAACGGCGCTTGGCGAGAGACTATGAACGTGCTTAAACTGGAGGGTGGC |
| psiCheck2- PRRSV-1666-MUT-R | GGCCGCCACCCTCCAGTTTAAGCACGTTCATAGTCTCTCGCCAAGCGCCGTTCCTGTC |
| psiCheck2- PRRSV-3249-MUT-F | TCGAGATCATGCGTGAGGCTTGTGATGCGTAGAGACTATGAGATCCTGCTACGCAGGGC |
| psiCheck2- PRRSV-3249-MUT-R | GGCCGCCCTGCGTAGCAGGATCTCATAGTCTCTACGCATCACAAGCCTCACGCATGATC |