Table 3. Summary of mutation analysis of NGS ALK fusion negative cases.
| FISH | Case # | FISH POSITIVE CELLS (%) | IHC | GENOMIC CHANGES | |
|---|---|---|---|---|---|
| MUTATIONS (% Mutant Allele Frequency) |
COPY NUMBER CHANGES | ||||
|
Fusion and split signal (N=3) |
1 | 20.5 | NEG |
KRAS p.G12V (6%) FGFR1 p.E126fs (97%) |
CDKN2A loss |
| 2 | 26.1 | NA | NA | NA | |
| 3 | 36.9 | NEG | ATM p.I346N (53%) | None | |
|
Fusion and single orange (N=6) |
4 | 76.6 | NEG | TP53 p.A161D (54%) | CDKN2A loss |
| 5 | 79.7 | POS | KRAS p.G12C (74%) | CDKN2A loss | |
| 6 | 87.3 | NEG |
TP53 p.G245C (50%); TP53 p.E294fs (24%) BRAF p.G593F (28%) |
CDKN2A loss KRAS gain EZH2 gain |
|
| 7 | 82.4 | NEG | N/A | NA | |
| 8 | 50.0 | NEG | EGFR p.L858R (84%) |
EGFR gain CDKN2A loss |
|
| 9 | 79.7 | POS | NA | NA | |
|
Mixed pattern (N=3) |
10 | 30.5 | NEG | TP53 p.G226R (92%) FGFR1 E126fs | CDKN2A loss |
| 11 | 20.7 | NEG | MET p.T1010I (48%); | CDKN2A loss | |
| 12 | 22.5 | NEG | CTNNB1 p.G34E (61%) |
RET loss RB1 loss |
|
NA- insufficient tumor tissue left for IHC or mutation analysis.