Figure 2. PRIMA-1^Met inhibited anchorage-independent growth of colorectal cancer cells and EGF-induced neoplastic transformation of JB6 P+ cells.

A-C., Representative images of colony formation. Cells were seeded in soft agar with the addition of different concentration of PRIMA-1^Met (0, 12.5, 25, 50 uM) and cultured for 7 days (SW480 and HCT116^neg) or 10 days (HCT116^wt), then colonies were counted. Data were shown as mean ± SEM from triplicate experiments. ^**P<0.01 vs. vehicle group. PRI: PRIMA-1^Met. D. JB6 P+ cells were cultured with or without EGF (10 ng/ml) and different concentrations of PRIMA-1^Met (0, 25, 50 uM) for 7 days, then colonies were counted. Data were shown as mean ± SEM from triplicate experiments. ^* P<0.05; ^**, P<0.01 vs. EGF single group. PRI: PRIMA-1^Met.