Table 1. Kinase profiling results of PRIMA-1Met.
| Kinase | Activity | Kinase | Activity |
|---|---|---|---|
| ALK | 90 | JNK3(h) | 96 |
| AMPKα1(h) | 94 | KDR(h) | 93 |
| Aurora-A(h) | 83 | MAPK1(h) | 75 |
| Aurora-B(h) | 91 | MAPK2(h) | 80 |
| B-Raf (h) | 80 | MEK1(h) | 35 |
| CDK1/cyclinB(h) | 94 | MEK2(h) | 48 |
| CDK2/cyclinA(h) | 102 | mTOR(h) | 86 |
| CDK3/cyclinE(h) | 73 | p70S6K(h) | 90 |
| C-MET(h) | 60 | PDK1(h) | 76 |
| C-RAF(h) | 92 | PKBα(h) | 110 |
| cSRC(h) | 67 | PKBβ(h) | 100 |
| EGFR(h) | 95 | PKCα(h) | 88 |
| FGFR1(h) | 83 | PKCδ(h) | 92 |
| GSK3α(h) | 105 | Rsk1(h) | 88 |
| GSK3β(h) | 110 | Rsk2(h) | 79 |
| IGF-1R(h) | 92 | Src(T341M)(h) | 110 |
| IKKα(h) | 94 | TGFBR1 (h) | 93 |
| IKKβ(h) | 90 | PI3K (p110α/p85α) (h) | 97 |
| JNK1α1(h) | 70 | PI3K (p110 β/p85α) (h) | 90 |
| JNK2α2(h) | 84 | PI3K (p110δ/p85α) (h) | 94 |
Kinase screening assay was performed according to Millipore's Kinase Profiler protocols. Briefly, the reaction concentration of PRIMA-1Met was set at 100 μM and the concentration of ATP at 10 μM. Scores for each kinase were represented as the percent (%) of kinase activity after PRIMA-1Met treatment.