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. 2016 Nov 15;7(50):83040–83050. doi: 10.18632/oncotarget.13349

Figure 1. Isolation of human anti-CD47 antibodies.

Figure 1

(A) SDS-PAGE analysis results of the purified antibodies (IgG) under non-reducing and reducing conditions. (B) Isolated antibodies bound to recombinant CD47 specifically analyzed by direct ELISA. The antigens from left to right were PBS (as blank control), VEGF, KDR, IFN-γ, PDL1, HER2, Casein, BSA, and CD47 for each antibody. (C) Isolated antibodies bound to recombinant CD47 in a dose-dependent manner by direct ELISA. (D) ZF1 bound to cell-surface CD47 in a dose-dependent manner by Flow Cytometry. B6H12 served as positive control antibody in all assays.