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. 2016 Nov 15;7(50):83040–83050. doi: 10.18632/oncotarget.13349

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Copyright: © 2016 Zeng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A, B and C) Representative results for phagocytosis of CFSE-labeled CCRF cells phagocytosed by Dye eFluor^® 670-labeled macrophages. The results were firstly recorded by image (A), then analyzed by Flow Cytometry (B) and showed in a bar graph (C). (D) Anti-CD47 antibodies induced phagocytosis of U937 by macrophage at dose-dependent manner. Human IgG and anti-EGFR antibody Cetuximab were set as negative control at 10 μg/ml. (E) ZF1 blocked interaction between recombinant human CD47and recombinant human SIRPα.