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. 2016 Nov 4;7(50):83060–83070. doi: 10.18632/oncotarget.13067

Figure 3. Src is a direct target gene of miR-31 and inversely correlated with miR-31 in GBC patients.

Figure 3

A. Sequence of the miR-31-binding site within the human Src 3′-UTR and a schematic diagram of the reporter construct showing the entire Src 3′-UTR sequence and the mutated Src 3′-UTR sequence. Src-WT: wild-type; Src-MUT: mutant type B. Luciferase assay of NOZ/DDP cells co-transfected with vector or miR-31 and a luciferase reporter containing the full length of Src 3′-UTR (WT) or a mutant (MUT). Luciferase activities were measured 24 hours post-transfection. Src-WT: wild-type; Src-MUT: mutant type C. The expression level of Src measured by qRT-PCR and normalized to GAPDH. D. The immunoblotting data of Src and p-Src(Y416) expression in DDP-resistant GBC cells transfected with miR-31 or vector. E. The mRNA expression of Src in tumor tissues and the adjacent non-tumor tissues from 41 GBC patients by qRT-PCR. F. An inverse correlation between miR-31 and Src mRNA expression by Pearson correlation analysis (Pearson's correlation: r= −0.56, P<0.001). Data were presented as mean ± SD. **P < 0.01.