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. 2016 Nov 4;7(50):83148–83159. doi: 10.18632/oncotarget.13074

Figure 3. IL-6 in ovarian cancer patient derived ascites increase migration and invasion of SKOV-3 cells via JAK2-STAT3 signaling.

Figure 3

A. SKOV-3 cancer cells were treated with 10% ascites that were either pre-treated with or without 50 ng/ml of IL-6 antibody (IL-6 Ab) for 6 hr. Recombinant IL-6 (10ng/ml) was used as a positive control. After 24 hr, wound healing ability was verified by measuring wound closed area under a light microscope (magnification x40). B. SKOV-3 cancer cells were seeded into the upper chamber of Matrigel-coated membrane in transwells. Cell invasion were induced by ascites as above. After 24 hr, invaded cells at the bottom of the transwell were stained with 0.5% crystal violet and counted under a light microscope (magnification x200). C. SKOV-3 cancer cells were treated with IL-6 Ab as above. After 24 hr, the expression of Snail, Vimentin, N-cadherin and E-cadherin were examined by western blot. GAPDH was used as an internal control. D. SKOV-3 cancer cells were treated with or without 10% ascites for 0 - 6 hr. The expression of p-JAK2 (Y1007), JAK2, p-STAT3 (Y705) and STAT3 were examined by western blot. GAPDH was used as an internal control. E. IOSE380 and SKOV-3 cells were treated with or without 10% ascites. After 0.5 hr, the expression of p-JAK2 (Y1007), JAK2, p-STAT3 (Y705) and STAT3 were examind by western blot. GAPDH was used as an internal control. F. SKOV-3 cancer cells were treated with IL-6 Ab as above. The expression of p-JAK2, JAK2, p-STAT3 and STAT3 were examined by western blot. GAPDH was used as an internal control. * and *** represent P < 0.05 and P < 0.001, respectively.