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. 2016 Nov 4;7(50):83148–83159. doi: 10.18632/oncotarget.13074

Figure 5. Expression of IL-6R on cell membrane correlates with ascites-induced invasion.

Figure 5

A. Basal mRNA expression of IL-6 receptor (IL-6R) in ovarian cancer cell lines were examined by RT-PCR. B. Basal protein expression of IL-6R in ovarian cancer cell lines was analyzed by western blot. α-tubulin was used as an internal control. C. Ovarian cancer cells were seeded into the upper chamber of Matrigel-coated membrane in transwells. Cell invasion were induced by ascites. After 24 hr, invaded cells at the bottom of the transwell were stained with 0.5% crystal violet and counted under a light microscope (magnification x200). D. Expression of IL-6R on cell membrane of ovarian cancer cells were examined by immunocytochemistry. The representative confocal microscopy figure (Green, IL-6R; red, plasma membrane; blue, DAPI) are shown. Original magnification 400x for all panels. E. Expression of IL-6R on cell membrane of ovarian cancer cell lines were examined by isolation of proteins from the plasma membrane and the cytosol fraction. The expression level of IL-6R was examined by western blot. Pan-cadherin and α-tubulin were used as an internal control. *, ** and *** represent P < 0.05, P < 0.01 and P < 0.001, respectively.