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. 2016 Nov 4;7(50):83148–83159. doi: 10.18632/oncotarget.13074

Figure 6. Silencing of IL-6R impairs ascites-induced invasion.

Figure 6

A. PA-1 and SKOV-3 cells were transfected with IL-6R targeted siRNA (100 nM) or scrambled RNA (100 nM) as a negative control. Silencing of IL-6R expression was examined by western blot. B. PA-1 and SKOV-3 cells were transfected with IL-6R targeted siRNA (100 nM) or negative control scrambled RNA (100 nM) for 24 hr. Cells were seeded into the upper chamber of Matrigel-coated membrane in transwells. Cell invasion were induced by 10% ascites. After 24, invaded cells at the bottom of the transwell were stained with 0.5% crystal violet and were counted under a light microscope (magnification x200). The data summarized in the bar charts are presented as mean ± SD of three independent fields. * and ** represent P < 0.05 and P < 0.01, respectively.