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. 2016 Nov 4;7(50):83187–83199. doi: 10.18632/oncotarget.13077

Figure 4. shRNA knockdown of CLIC1 confirms that CLIC1 influence proliferation, resistant to hydrogen peroxide and cisplatin.

Figure 4

A. Western blotting was used to validate the efficiency of CLIC1 knockdown in A2780-CLIC1 KD cells; B. q-PCR was used to measure the relative repression ratio of CLIC1 mRNA compared to GAPDH as an internal control in A2780-NCi and A2780-CLIC1 KD cells; C. knockdown of CLIC1 in A2780 reduces the growth rate of cells, and cells were observed every 12 h; D. the measurement of cell cycle in A2780 NCi and A2780-CLIC1 KD cells; E. percentages of variable cells treated with hydrogen peroxide at different concentrations for 24 h determined with CCK-8 assay; F. percentages of variable cells treated with cisplatin at different concentrations for 24 h determined with CCK-8 assay. A2780-CLIC1 KD, A2780 cell line with CLIC1 knockdown; A2780-NCi, A2780 cell line with knockdown control shRNA NCi. ***p<0.001; NS, no significance; n=3.