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. 2016 Nov 4;7(50):83187–83199. doi: 10.18632/oncotarget.13077

Figure 5. shRNA knockdown of CLIC1 leads to the down regulation of CTPS1, the decrease of CTP concentration and the arrest of G1 phase.

Figure 5

A. q-PCR was used to confirm the down regulation of CTGF, GLUD2, CMPK1, DSP, CTPS1 and VCL on mRNA level; B. Western blotting was used to confirm the down regulation of proteins CTPS1; C. Metabolomics was used to measure the concentration of CTP in A2780-CLIC1 KD and A2780-NCi cells. A2780-CLIC1 KD, A2780 cell line with CLIC1 knockdown; A2780-NCi, A2780 cell line with knockdown control shRNA NCi. ***p<0.001; **p<0.01; n=3.