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. 2016 Nov 4;7(50):83231–83240. doi: 10.18632/oncotarget.13081

Figure 2. Build HUVECs IR model.

Figure 2

A-C. Culture HUVECs by low glucose DMEM complete medium under conditions of low glucose(LG), low glucose combined with high insulin(LG+INS), high glucose(HG), high glucose combined with high insulin (HG+INS) for 24 hours. The expression of IRS1, PI3K and p-Akt were detected by Western blotting analysis. *P < 0.05 compared with control. D-F. Culture HUVECs under high glucose combined with high insulin for 0, 2h, 6h, 12h, 24h,the expression of IRS1,PI3K and p-Akt were detected by Western blotting analysis. *P < 0.05 compared with 0h. Data are means ± SD from at least three separate experiments.