Figure 2. FLO-1 exhibits a mesenchymal phenotype.

(A–B) Representative H&E staining of primary FLO-1 xenografts demonstrating (A) poor tumor differentiation and (B) lymphovascular invasion (LVI, arrows). (C) Summary of tumor differentiation and LVI status for all cell line xenografts. (D) Comparison of endogenous mRNA expression of EMT genes across all cancer cell lines. mRNA expression for each cancer cell line is normalized to NES cells (Broken line), a normal esophageal squamous cell line. One-way ANOVA with Dunnett's multiple comparison posttest. Error bars = SEM, n = 2, *p < 0.05, ***p < 0.001. (E) Representative western blots of the indicated protein (Above) and their associated densitometric analysis (Below, normalized to β-actin) in all cancer cell lines. (F) Representative E-cadherin immunohistochemistry staining in all cancer cell line xenografts. See also Supplementary Figure S2.