Figure 4. FGFR signaling is similarly increased in acquired and inherent drug resistance.

A. Gene expression was analyzed in parental Her2-transformed HMLE cells, the post-TGF-β cell population, and Lapatinib resistant (LapR) cells using the Nanostring PanCancer Progression panel. Plot shows the fold change in expression of the 770 genes in the panel for the post-TGF-β cells (X-value) and LapR cells (Y-value) normalized to parental values as 1 (dashed lines). This comparison resulted in the indicated coefficient of determination (r²). B. Selected data from the Nanostring panel showing similar gene expression changes in the indicated EMT transcription factors, FGF receptors and FGF ligands. C. Expression changes in these factors were confirmed by RT-PCR analysis. Data are normalized to parental HMLE-Her2 values and are the mean ± the SD of three independent samples.