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. 2016 Nov 7;7(50):83530–83543. doi: 10.18632/oncotarget.13171

Figure 5. ACDB induces the activation of caspase in human chondrosarcoma cells.

Figure 5

A. JJ012 cells were incubated with ACDB for 24 h. Caspase-3/7 activities were examined by caspase activity kit. B. JJ012 cells were incubated with ACDB for different time intervals. Levels of PARP, cytochrome C, caspase-3, caspase-7 and caspase-9 expressions were examined by Western blot analysis. Results are expressed as the means ± SEM of four independent experiments. C. Cells were pretreated for 30 min with z-DEVD-FMK (caspase 3 inhibitor) or z-LEHD-FMK (caspase 9 inhibitor), followed by stimulation with ACDB for 24 h. The percentage of apoptotic cells was the analyzed by flow cytometry of PI-stained cells. *P < 0.05 as compared with control group; #P < 0.05 compared with the ACDB-treated group.