Table 2.

Differences between mouse and rhesus models

Parameter	Mouse model	Rhesus model
Priming vaccination (Table 1; Fig. 2)	~900-fold higher Qβ-CSP NANP titres after first dose	~12-fold difference between vaccines
Booster vaccination (Fig. 2)	Titres were boosted by each vaccination	No boosting beyond second dose
Immunogenicity (Table 1; Figs. 2, 6)	Significantly higher FL and NANP titres for Qβ-CSP group after the third dose	Differences between vaccines was lower and not significant
Serum antibody avidity (Fig. 3)	Higher avidity of Qβ-CSP group	No difference in avidity
NANP epitope bias (Fig. 4)	NANP-biased Qβ-CSP group response after each dose	NANP-biased response of Qβ-CSP after 1st dose only
NANP mAb competition (Fig. 5)	Higher mAb 2A10 inhibition by anti-Qβ-CSP	No difference in mAb inhibition detected
N-terminal response (Fig. 5)	Higher N-terminal response by Qβ-CSP	No difference in N-terminal response
Functional assay (Fig. 6)	No difference in protection observed after direct challenge	Qβ-CSP protected more mice in a neutralization assay

Summary of observed differences between animal models in the present study, with respect to comparison between Qβ-CSP and CSP vaccines. Tables and Figures illustrating noted differences are referenced for each parameter