Table 4.

Binding affinities for complex 2 to cyt c in different buffers. All buffers were at 5 mm concentration, pH 7.5.

Buffer	K d [nm]	Buffer	K d [nm]
sodium phosphate	42.9±3.1	HEPES	31.2±3.1
potassium phosphate	26.2±3.1	Tris	106.3±32.6
MOPS	35.2±3.1	btp	133.5±37.4