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. 2017 Feb 27;69(3):565–575. doi: 10.1002/art.39965

Figure 3.

Figure 3

Higher molecular weight (Mwt) species of phosphorylated transforming growth factor β–activated kinase 1 (TAK‐1) in injured porcine and murine cartilage. A, Porcine metacarpophalangeal joints were processed as described in Figure 1. Cartilage was then dissected and either snap‐frozen (time point 0 minutes) or cultured for 5 or 10 minutes. Cartilage lysates were analyzed by Western blotting for TAK‐1 phosphorylated at T187 (ph‐T187), T184/T187, and S192, phospho‐JNK, and total ERK (T‐ERK; loading control). B, Murine proximal femoral epiphyses (2 per time point) were subjected to avulsion injury, and were then either snap‐frozen (time point 0 minutes) or cultured for 5 or 10 minutes in serum‐free medium. Epiphyses were analyzed by Western blotting for phospho–TAK‐1, phospho‐JNK, and total ERK (loading control) as described in A. Representative results from 4 independent experiments are shown. NS = nonspecific.