Table 1.

mCitrine‐RGD protein constructs and characteristics.

GRGDS position	Cloning[a]	Fluorescence[b]	ELISA signal intensity [a.u.]
			αvβ3 [c]	αvβ5 [c]
none	+	398	0.058±0.010	0.123±0.003
Inserted
N terminus	+	n.a.	0.137±0.014	0.133±0.008
49/50	+	445	0.238±0.008	0.146±0.006
78/79	+	342	0.098±0.002	0.142±0.006
116/117	+	425	0.332±0.013	0.249±0.009
134/135	+	n.a.	0.480±0.050	0.136±0.013
140/141	+	393	1.60±0.05	0.98±0.10
157/158	+	372	0.164±0.002	0.142±0.001
172/173	+	393	0.445±0.024	0.180±0.010
189/190	+	377	0.313±0.008	0.195±0.004
194/195	+	240	1.90±0.04	0.789±0.025
213/214	+	405	0.629±0.054	0.135±0.006
C terminus	+	392	0.057±0.010	0.142±0.005
Exchanged
49/53	+	270	0.057±0.010	0.158±0.003
78/82	+	38	0.177±0.006	0.483±0.025
116/121	+	n.a.	0.124±0.003	0.125±0.003
135/138	+	n.a.	0.64±0.04	0.207±0.044
140/144	+	n.a.	0.63±0.02	0.439±0.014
156/160	+	479	0.244±0.009	0.164±0.010
171/174	+	392	0.244±0.011	0.188±0.030
190/194	−	n.d.	n.d.	n.d.
194/198	+	235	1.158±0.019	0.69±0.01
211/215	+	192	0.095±0.005	0.128±0.009

[a]+: successful; −: not successful. [b] Fluorescence intensity (arbitrary units): λ _ex=515 nm, λ _em=528 nm for 1 μm mCitrine‐RGD in PBS; n.a. not active; n.d. not determined. [c] ELISA signal intensity for two representative integrins. Values are mean±SD (n=3).