Figure 1.

Neutralization of host acidic compartments during Listeria infection. Acridine orange‐stained HeLa cells were infected for 1 or 5 hr with wild‐type (WT) or Δhly Listeria monocytogenes . (a) Representative flow cytometry analysis of uninfected or infected acridine orange‐stained HeLa cells. (b) Data represent the geometric mean of acridine orange fluorescence in infected cells normalized to that of uninfected cells, as measured by flow cytometry analysis (mean ± SD from at least three independent experiments; *, p < 0.05; unpaired two‐tailed Student's t test)