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LLO‐induced neutralization of acidic compartments is calcium‐independent. Geometric mean of acridine orange fluorescence, as measured by flow cytometry analysis, in HeLa cells pre‐incubated with 1 or 10 mM EGTA, or with 0.1 mM BAPTA‐AM, and treated with 3 nM LLO (mean ± SD from at least three independent experiments)
