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(A) A sample with a known EGFR exon 19 deletion mutation. (B) A sample with EGFR exon 21 mutation (p.L858R) is present (39% T > G transversion). (C) Sequencing result of EGFR exon 19 wild-type case with lack of in frame deletion. (D) A sample with wild-type EGFR exon 21 "hot-spot" position (100% T, no T >G transversion). Using the Integrative Genomics Viewer (IGV) software, we were able to determine the mutation and fraction of mutant copies.
