Fig 5. Tet represses cell migration and invasion of human RCC by negatively regulating NF-κB expression.

The metastatic phenotype of 786-O and 769-P was determined by transwell assay. The migrated and invaded 786-O (A) and 769-P (B) cells were counted after treatment with Tet (0.5μM), PDTC (10μM), or the both for 24 h. Under similar treatment, lysates from 786-O (C) and 769-P (D) cells treated with Tet or PDTC were immunoblotted for MMP-9 and NF-κB. All the experiments were performed in triplicate. Representative results from three independent experiments were shown.