Fig 1. ASO-mediated depletion of NEAT1 partially rescued CX5461-induced transcriptional inhibition.

A. qRT-PCR of the levels of NEAT1 lncRNA in HeLa cells either mock-treated or transfected with NEAT1 or control ASOs. B. Dose-dependent reduction of 47S rRNA precursor by CX5461 was partially rescued in NEAT1-depeted cells. C. Nascent rRNA was visualized by IF staining of incorporated 5-FU. HeLa cells were transfected with 30 nM cy3-labled ASOs and treated with DMSO or CX5461 (120 nM) for 2.5 hrs. Cells were pulsed with 1 mM 5-FU for 10 mins.