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. 2017 Mar 13;12(3):e0173494. doi: 10.1371/journal.pone.0173494

Fig 2. ASO-mediated depletion of NEAT1 alleviated the RNAP I inhibition induced nucleolar disruption.

Fig 2

A. IF showed the localization of fibrillarin to nucleoli (red arrows) in DMSO-treated HeLa cells. B. Fibrillarin redistributed to nucleoplasmic foci (the green box) or perinucleolar caps (red arrows) by CX5461. C. NEAT1 ASOs had no effect on the distribution of fibrillarin in DMSO treated cells. Cy3-labled NEAT1 ASOs (30 nM) were transfected for 2 hrs, followed by DMSO treatment for 2.5 hrs. D. Redistribution of fibrillarin to nucleoplasmic foci by CX5461 was prevented in cells containing NEAT1 ASOs (the red box). HeLa cells were transfected with 30 nM NEAT1 ASOs for 2 hrs, followed by CX5461 treatment (250 nM) for 2.5 hrs. E. Alleviated CX5461-induced nucleolar disruption was specific for NEAT1 depletion. HeLa cells were treated as described in Fig 2D with multiple NEAT1 ASOs or control ASOs. F and G. Combined NEAT1-FISH and IF of fibrillarin in HeLa cells. HeLa cells were treated as described in Fig 2D with either control ASO (F.) or NEAT1 ASO-1 (G.). Cells that contain enriched amount of ASOs were boxed in red. Cell that contain little or no ASOs were boxed in green.